Measurement of fractional whole-body gluconeogenesis in humans from blood samples using [sup.2]H nuclear magnetic resonance spectroscopy

Measurement of fractional whole-body gluconeogenesis in humans from blood samples using [sup.2]H nuclear magnetic resonance spectroscopy
Roden, M.
10/1/2003
Diabetes
Several problems limit quantification of gluconeogenesis. We applied in vitro [sup.2]H-nuclear magnetic resonance (NMR) spectroscopy to simultaneously measure [sup.2]H in all glucose carbons for direct assessment of gluconeogenesis. This method was compared with [sup.2]H measurement in carbons 5 and 2 using gas chromatography-mass spectrometry (hexamethylenetetramine [HMT]) and with in vivo [sup.13]C magnetic resonance spectroscopy (MRS). After 14 h of fasting, and following [sup.2][H.sub.2]O ingestion, blood was obtained from nine healthy and seven type 2 diabetic subjects. Glucose was ...
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